February 3, 2023
This document specifies the principle, materials, test methods, calculation of results, and quality of the determination of nitric oxide (NO) release inhibition in macrophages in vitro Quantitative control, outcome evaluation and conclusions. This document provides a test method for cosmetics and raw materials to inhibit LPS induced in vitro macrophage release of nitric oxide (NO). Claims of soothing efficacy of cosmetics and ingredients are backed by science. This document is intended for samples that achieve a soothing effect through an inflammatory response pathway caused by an external stimulus, not through other pathways Samples that cannot be evenly dispersed in aqueous solution, cannot penetrate the skin barrier, or have low test concentrations due to high cytotoxicity.
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